Table 1. Primers used for Real-Time PCR analysis of a few genes of Escherichia coli.

Sl.No.	Gene	Primer	Sequence (5′-3′)
1	hyaE	hyaE-rt F	CTTGACGACTGGCTTACG
		hyaE-rt R	GCCACCTGCCATGTATAG
2	mdtD	mdt-rt F	TTATCGTCGGGTACTGGTAG
		mdtD -rt R	GAGTTGACCATCCCTTGTAA
3	srmB	srmB-rt F	AACATATTGCTGGCGAAA
		srmB-rt R	GAGGGATTGGCAGAAACT
4	pyrD	pyrD-rt F	GAAGAATTGATCCAGGTTGC
		pyrD-rt R	TCCCTGAACAAGAGAACGAT
5	rhaD	rhaD-rt F	GCGAATGTTTTTGCATCTCT
		rhaD -rt R	CGAGTGTCTGGTGGTATTC
6	yicL	yicL-rt F	CGTCGCAGTTTTTGACTATG
		yicL -rt R	AAAAATCAGCAGGCTAATGG
7	ldcC	ldcC-rt F	TGTTGATGCCTGGAGAAA
		ldcC-rt R	TCAAAACCGGGGTAATGT
8	16S	16S-rtF	GTGCAATATTCCCCACTGCT
		16S-rtR	CGATCCCTAGCTGGTCTGAG