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. 2013 Mar 5;8(3):e58035. doi: 10.1371/journal.pone.0058035

Table 1. Process summary of WT CA purification by polymerization.

Sample Conc (mg/mL) Vol (mL) Total protein (g) % Purity1 CA yield (g)
Total cell lysate 28.1 6000 168.8 15.5 26.2
ammonium sulfate fraction 7.9 4800 38.1 69.1 26.3
1st polymerized fraction 11.2 1600 17.9 94.4 16.9
2nd polymerized fraction 24.7 485 12.0 98.0 11.7
Q-sepharose flow-through 21.3 490 10.5 98.3 10.3
1

determined using HT Protein Express LabChip in Caliper LabChip GXII system.