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. 2013 Mar 5;8(3):e57785. doi: 10.1371/journal.pone.0057785

Figure 3. ISH for SIV RNA in peripheral LN from SM and PM.

Figure 3

ISH was performed using short-fixed paraffin embedded tissue sections of peripheral lymph node collected at 2, 6 and 24 wpi in SM and 2, 5 and 21 wpi in PM. A, SIV-infected cells were not observed in GC of immune competent SM but were readily apparent in GC of PM at all time points. Diffuse hybridization signal characteristic for the presence of immune-complexed virions trapped by FDC was not observed in SM, but was identified in PM at all time points, with the most significant trapping seen at 5 and 21 wpi. B, Despite higher tissue viral burden in CD8 depleted SM compared to PM, germinal centers typically lack infected cells and virus trapping despite close proximity to numerous infected cells in the lymph node paracortex. C, GC in CD8-depleted SM at 2, 6 and 24 wpi. Only one infected cell was localized within a GC from a SM at any given time point in CD8-depleted SM. Virus trapping was also extremely rare in CD8 depleted SM (bottom).