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. 2013 Mar;23(3):440–451. doi: 10.1101/gr.142703.112

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© 2013, Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported License), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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Figure 1. — Sequential targeting of epitope-tagged H3.3 to chromatin. (A) Intranuclear localization of H3.3-mC 24 h after transient transfection. (B) Intranuclear localization of H3.3-mC and H3.2-EGFP after cotransfection as in A. (C) Percentage of cells shown in A displaying indicated H3.3-mC distribution patterns over time after transfection (mean ± SD of three experiments, with more than 120 cells analyzed per experiments). (D) Time-lapse imaging of H3.3-mC starting 24 h after transfection (see Supplemental Movie 1). Scale bars, 10 μm.