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. 2013 Mar;23(3):497–508. doi: 10.1101/gr.149112.112

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© 2013, Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported License), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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Figure 1. — Identification of small RNAs and associated proteins in Caenorhabditis species. (A) Bar graphs show the size and 5′ first nucleotide distributions of small RNAs. (Insets) Pie charts display the proportion of reads belonging to different classes of small RNAs: miRNAs, piRNAs, and siRNAs, or unclassified. (B) Phylogenetic relationship of the elegans group of Caenorhabditis, adapted from Kiontke et al. (2011). C. briggsae, C. remanei, C. brenneri, and C. elegans (highlighted in red), having had their genomes sequenced and partially annotated, were chosen for this study. (C) Phylogeny of RNA-dependent RNA polymerases. Genes from different species are color-coded, and different subfamilies are indicated by colored branches. (Cel) C. elegans, (Cbr) C. briggsae, (Cre) C. remanei, (Cbn) C. brenneri. (D) RT-PCR of rrf-4 from mixed-stage C. briggsae, C. remanei, and C. brenneri.