Figure 6.

Savant screen shot showing healthy breast (from Illumina BodyMap2) and triple-negative breast cancer RNA-seq data. The third and fourth tracks display the aligned reads from healthy and cancer tissue, respectively, with the colors representing the isoform of origin. (Red isoform) Canonical annotated isoform. Its presence may be due to healthy cells biopsied together with the tumor. (Green isoform) Pre-mRNA (or partially spliced RNA); (blue) contains the alternative acceptor site; (yellow) skips the next exon (to the left since the transcript is on the reverse strand). We can also see the single nucleotide variant (SNV) that disrupted the acceptor site of the intron.