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. 2013 Mar;23(3):530–538. doi: 10.1101/gr.143693.112

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© 2013, Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported License), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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Figure 5. — Influence of “disruptive” interfinger linkers on extended-MA ZFN activity. ZFN activity was determined by the SSA assay for ZFNs with long linkers (TGSQKP) inserted between fingers 2 and 3 and fingers 4 and 5. (Std Link) All canonical linkers (TGEKP). The array configuration for the Std Link ZFN was GZF3-L3 + GZF1-R3, CS2-1 L5 + R3, CS5-1 L4 + R4, CS6-2 L5 + R4, CS7-3 L4 + R6, and T2-X6 L5 + R4.