Fig. 4.

The CLSM photographs of non-fixed tissues. Transportation of 6(5)carboxyfluoresceine (6(5)CF) from mesophyll of leaflet (a) into root (b) and root nodule (c, d, e). Notice the fluorescence of vascular bundles (arrows in d, e). The control root nodule exhibits only the pale autofluorescence of uninfected cells (double arrowhead in f). Abbreviations: IT – inner tissue, M – meristem, PC – pericycle cells, SZ – symbiotic zone, TE – tracheary elements, VE – vascular endodermis, VB – vascular bundle. 6(5)CF fluorescence was observed after excitation at 488 nm, emission spectrum was between 500 nm and 566 nm. Bars represent: A = 115 μm, B = 250 μm, C = 100 μm, D = 60 μm, E = 20 μm, F = 150 μm