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Alignment of PsaE proteins from two Paulinella strains. PsaE from FK01 strain is equipped with an N-terminal extension (yellow box) that was predicted with high confidence as a signal peptide by 27 from 30 algorithms with an unambiguous cleavage site (Mackiewicz and Bodył 2010). Eight non-conserved substitutions according to Blosum62 matrix (in red), which change biochemical properties of residues, may constitute some internal PsaE targeting signal in the CCAC0185 strain
