Figure 6.

Knockdown of endogenous Msx1 in αT3-1 cells increases endogenous mouse αGSU and GnRHR mRNAs. (A) αT3-1 cells were transfected with SMARTpools of siRNA for Msx1 or a scrambled control. Cells were transfected for 72 hours, at which point total RNA were harvested and reverse transcribed. The resulting cDNA was used in qPCR reactions with primers specific for Msx1, αGSU, GnRHR, and GAPDH. All values represent the SQ means from 4 independent experiments with all means adjusted to corresponding GAPDH values within experiment. All values are normalized to scrambled control. (B) Whole-cell lysates were used for protein expression by Western blotting. An asterisk indicates significant differences (P < .05) from empty vector control.