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Hyperinsulinemia-induced endogenous Sp1-DNA–binding activity is prevented by DON. ChIP was performed on 3T3-L1 adipocytes treated with or without 500pM insulin (Ins) and/or 20μM DON. Purified DNA and primers specific to the Sp1-binding site in the promoter region of Srebf1 were used for qPCR. Ct values from qPCR were normalized to the background IgG antibody using the fold enrichment method. Values are mean ± SEM from 3 independent experiments. *P < .05 vs control.
