Table 1.
Primer | Sequence (5′–3′) |
---|---|
pGL2B-Hmgcr | |
Forward | TAGGTACCCATCCCCTGTTCCCCGCG |
Reverse | TAAAGCTTGTCTCCAGCCAACGGAGC |
ChIP-Hmgcr | |
Forward | ACCCGTCATTGGTTGGCTCT |
Reverse | CTCCCTAACAACCGCCAACT |
ChIP-Srebf1 | |
Forward | CCATCCCTGGCCCTTTAATCTAACGA |
Reverse | TTCGGACTAGGCCCACGTTAAGGAAA |
qPCR-Hmgcr | |
Forward | TGTGGGAACGGTGACACTTA |
Reverse | CTTCAAATTTTGGGCACTCA |
qPCR-36B4 | |
Forward | AAGCGCGTCCTGGCATTGTCT |
Reverse | CCGCAGGGGCAGCAGTGGT |
Forward and reverse represent a pair of sense primers and antisense primers, respectively.