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. 2013 Jan 22;27(3):511–523. doi: 10.1210/me.2012-1307

Figure 2.

Figure 2.

IGF-I does not affect FSHR expression in GCs in culture. Rat GCs were cultured in the presence of FSH (50 ng/ml) and/or IGF-I (50 ng/ml) for 48 hours. Cells were harvested for protein or RNA isolation, and the culture medium was collected for IGF-I determination. A, FSHR and β-actin (BACT) protein levels were quantified by using Western blot. B, Rat GCs were cultured for 15, 24, or 48 hours before FSHR mRNA quantification. C and D, IGF-IR (C) and IGF-I (D) mRNA expression levels after treatment for 48 hours as indicated in the graph. E, IGF-I levels were quantified by ELISA after 48 hours treatment with FSH. F, Rat GCs were cultured in the presence of forskolin (Fsk, 5μM) or dbcAMP (2mM) with or without IGF-I (50ng/ml) for 48 hours before Cyp19 mRNA quantification. Columns with different letters differ significantly (P < .05). Error bars are ±SEM; n = 3.