Figure 4.

In vivo IGF-IR inhibition blocks FSH-induced follicle growth. AEW (50 μg per ovary) or vehicle (control) was injected into the ovarian bursa of immature rats 2 hours before the administration of 10 IU eCG, and 48 hours later, one ovary was processed for hematoxylin and eosin staining and the other was used to isolate total RNA for mRNA quantification. A, Hhistology of a representative ovary (bar, 0.5 mm). B, Mean and SEM of relative mRNA levels. *** P < .01 vs control. Three animals were included in each group.