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Effect of PRL-dependent pTyr-PAK1 on closure of wounded monolayer. Monolayers of T47D cells stably overexpressing GFP, PAK1 WT, or PAK1 Y3F were wounded and cultured without (white bar) or with (black bar) PRL. Bars represent mean ± SE. * P < .05 compared with the same cells untreated with PRL. Each experiment was repeated three times with 10 measurements each time. n = 3 for each experimental condition. Scale bar, 200 μm.
