Table 1.
Experimental conditions for hippocampal STDP at Schaffer collateral CA1 synapses in the hippocampus.
| Study | Type of hippocampal preparation | Preparation condition | Species | Age | STDP protocol pre: post (current injection); repeat numbers with frequency or duration | Special conditions | t-LTP |
|---|---|---|---|---|---|---|---|
| Debanne et al., 1994 | Organotypic slice culture | Roller-tube culture | Rat | 2–4 weeks (DIV) | 1:>1 (240 ms, 0.5–2.0 nA); 50–100× ~0.3 Hz | Yes* | |
| Debanne et al., 1996 | Organotypic slice culture | Roller-tube culture | Rat | DIV>14 | 1:6–12 (240 ms, 0.5–2.5 nA); 50–100× at 0.3 Hz | Paired recordings | Yes* |
| Magee and Johnston, 1997 | Acute slice | ACSF/sucrose? | Rat | 5–10 weeks | 5 × 5:5 × 1–3 (2 ms, 2 nA at 5 Hz); 2× at ~0.07 Hz | 0.01 mM bic | Yes |
| Bi and Poo, 1998 | Dissociated cell culture | Rat | DIV 8–14 | pre:1 (1 ms, −70 to +30 mV); 60× at 1 Hz (post: suprathreshold EPSP response) | Paired recordings | Yes | |
| Pike et al., 1999 | Acute slice | ACSF | Rat | Young adult 120–200 g | 1:1 (5 ms, 1 nA); 10× at 5 Hz | 10 μM bic | No |
| 3:>1 (20 ms, 1 nA); 10× at 5 Hz | Yes | ||||||
| Nishiyama et al., 2000 | Acute slice | ACSF | Rat | 26–33 days | >1 (5 Hz):>1 (2 ms, 2 nA at 5 Hz); 16 s | Yes | |
| Mouse | 20–23 days | ||||||
| Meredith et al., 2003 | Acute slice | ACSF/sucrose? | Rat | 9–45 days | 1:1 (5 ms, 0.1–0.85 nA); 30× at 0.2 Hz | 9–15 days | Yes |
| Mouse | 1:1 (5 ms, 0.1–0.85 nA); 30× at 0.2 Hz | 22–43 days | No | ||||
| 1:1 (5 ms, 0.1–0.85 nA); 30× at 0.2 Hz +5 μM bic | 30–45 days | Yes | |||||
| 1:>1 (20 ms; 0.1–0.85 nA); 30× at 0.2 Hz | 22–43 days | Yes | |||||
| Wittenberg and Wang, 2006 | Acute slice | ACSF | Rat | 14–21 days | 1:1 (3 ms, 1.2–2 nA); 70–200× at 0.1–5 Hz | 100 μM PiTX | No |
| 1:2 (20–30 ms or 2*3 ms, 1.2–2 nA); 70–100× at 5 Hz | Yes | ||||||
| Remy and Spruston, 2007 | Acute slice | ASCF | Rat | 3–5 weeks | pre: 5 (100 Hz) inducing dendritic spikelets | 4 μM SR95531 | Yes* |
| pre: 5 (100 Hz) without inducing dendritic spikelets | 1 μM GCP52432 | No | |||||
| Harvey and Svoboda, 2007 | Acute slice | Choline chloride | Mouse | 14–18 days | 1 uncaging stimulus:3 (2 ms, 1–3 nA, 50 Hz); 60× at 2 Hz | Yes | |
| Organotypic slice culture | Stoppini-culture | Rat | DIV 7–11 | ||||
| Gerkin et al., 2007 | Dissociated cell culture | Rat | DIV 10–15 | 1 (1–2 ms,100 mV):1 (1–2 nA, 2 ms); 60× at 1 Hz | Paired recordings | Yes | |
| Campanac and Debanne, 2008 | Acute slice | Sucrose-based ACSF (sodium-free) | Rat | 15–20 days | 1:1 (3–5 ms, 300–500 pA); 100× at 0.33 Hz | 100 μM PiTX | Yes |
| Carlisle et al., 2008 | Acute slice | ACSF | Mouse | 6–12 months | 1:1 (5–7 ms, 0.5–1 nA); 100× at 5 Hz | 100 μM PiTX | No |
| 6–12 weeks | 1:3–4 (50 ms, 0.5–1 nA); 100× at 5 Hz | Yes | |||||
| Tanaka et al., 2008 | Organotypic slice culture | Stoppini | Rat | DIV 8–12 | MNI-glutamate uncaging (80 s at 1 Hz):1 (2 ms, 1–2 nA) | Yes | |
| Hardie and Spruston, 2009 | Acute slice | ACSF | Rat | 4–8 weeks | 1:1–3 (5 ms, 1.5–3 nA); 40× at 5 Hz | 4 μM SR95531 | Yes |
| weak input: synaptically driven action potentials (1–3) | 1 μM GCP52432 | Yes | |||||
| Zhang et al., 2009 | Cell culture | Rat | DIV 9–15 | 1:1 (2 ms, 1–2 nA); 60× at 1 Hz | Paired recordings | Yes | |
| Edelmann and Lessmann, 2011 | Acute slice | ACSF | Rat | 15–20 days | 1:1 (2–3 ms, 1 nA); 100× at 0.5 Hz | 100 μM PiTX | Yes |
| Acute slice | Sucrose-based ACSF (sodium-free) | Rat | 15–20 days | 1:1 (2–3 ms, 1 nA); 100× at 0.5 Hz | 100 μM PiTX | No | |
| 1:2 (each AP: 2 ms, 1 nA); 50× at 0.5 Hz or 70× at 5 Hz | |||||||
| 5:5 (each AP: 2 ms, 1 nA); 15–30× at 0.2 Hz |
The table compares preparation type, condition, species, age, and STDP protocol as well as the respective results in terms of induction of t-LTP. For interpretation of STDP protocol: 1:1 (2–3 ms, 1 nA); 100× at 0.5 Hz denotes one presynaptic stimulation (by extracellular stimulation, exceptions indicated) paired with one postsynaptic stimulation (induced by a 2–3 ms long 1 nA somatic current injection) repeated 100× at 0.05 Hz.