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. 2013 Mar 6;4:38. doi: 10.3389/fmicb.2013.00038

FIGURE 2.

FIGURE 2

Localization of GFP-fused CR-1 and CR-2 in epidermal cells of N. benthamiana leaves. Proteins were expressed by agroinfiltration and visualized at 48 h post infiltration by confocal laser scanning microscopy. (A) Co-expression of GFP:CR-1 with the red fluorescent marker protein mCherry, which localizes to the cytoplasm and the nucleoplasm in plant cells (Lee et al., 2008). (B) and (C) Co-expression of GFP:CR-2 with mCherry in two individual cells. Arrows indicate the motile CR-2 globules revealed in frame captures. (D) Co-expression of GFP-CR-2 with YFP-Tal (red channel), a fluorescent marker for actin cytoskeleton (Shemyakina et al., 2011). (E) Expression of ER-mRFP, the protein targeted to the ER lumen by N-terminal signal peptide and C-terminal ER-retention signal (Haseloff et al., 1997), in the perinuclear region of a plant cell. (F–H) Co-expression of GFP:CR-2 with ER-mRFP. (F) Perinuclear groups of GFP:CR-2-containing globules. (G) Modified perinuclear ER representing diffuse membrane reservoirs. (H) Overlap of images (F) and (G). All images represent the superpositions of series of confocal optical sections. N, nucleus. Scale bar, 10 μm.