Figure 3. The Effect of RSV on the Induction of Apoptosis in Cancer Cell Line, A431. Cells were infected with different concentrations of RSV (MOI of 1 and 3) for 36 (A) and 48 (B) hours. Annexin-V and PI staining methods were employed for detection of apoptosis using flow cytometry. The percentages of early and late apoptotic cells were increased following exposure to RSV in a concentration- and time-dependent manner. The results (Mean ± SD) represent at least 3 separate experiments. Differences between RSV infected and non-infected cells were statistically analyzed by ANOVA (* = P < 0.05, **= P < 0.01, ***= P < 0.001).
