. Author manuscript; available in PMC: 2014 Feb 26.

Published in final edited form as: Biochemistry. 2013 Feb 14;52(8):1364–1372. doi: 10.1021/bi400013k

Table 2. Oxygenation of fatty acids by Ath α-DOX.

Oxygenase activity was measured using an oxygen electrode with the fatty acids LA, αLA, and OA. K_cat and K_M values were derived for each substrate using triplicate measurements.

Substrate	k_cat (s⁻¹)	K_M (μM)	k_cat / K_M

αLA (18:3 ω-3)	11 ± 1.1	36 ± 3.2	0.31
LA (18:2 ω-6)	12 ± 0.4	23 ± 2.0	0.52
OA (18:1 ω-9)	11 ± 0.3	15 ± 1.9	0.73