Figure 4. Canonical Notch pathway proteins are expressed during axon targeting.
(A–A‴) Expression of Delta (red) and Notch (green) in GFP-labeled DCNs (blue) at ∼32 hr APF. DCNs express both Delta and Notch and the proteins appear intracellular, indicative of active signaling. Scale bar: 5 µm. (B, B′) Expression of Neur-LacZ (red) in GFP-labeled DCNs (green) at ∼32 hr APF. (C–F) Expression of E(spl)-CD2 reporter (red) in the DCN soma at ∼32 hr APF. Within the dorsal cluster, there are DCN soma with different E(spl) expression levels (marked with white arrows). (C′–F′) Expression pattern of E(spl)-CD2 reporter (red) together with GFP. White arrows indicate DCN soma with little or no E(spl)-CD2 expression. Scale bar: 5 µm. DCN: dorsal cluster neurons.