The effects of Gefitinib on the phosphorylation of epidermal growth factor receptor (EGFR), AKT, and ERK and expression of BIM in NSCLC cell lines. (A) PC-9 cell. (B) A549 cell. (C) SPC-A1 cell. (D) NCI-H1975 cell. Tumor cells were seeded in six-well plates and treated with Gefitinib for 24 hours, and then cells were lysed, and the lysate was subjected to SDS-PAGE, and probed with indicated antibodies.