FIG. 3.

The role of BIM in the the apoptosis induced by Gefitinib in PC-9 cells. (A) BIM silencing in PC-9 cells. PC-9 cells were seeded in 6-well plate, BIM-specific, and control small interfering RNAs (siRNA) were transfected with Lipofectamine 2000. After transfection, cells were lysed, and the lysate was subjected to SDS-PAGE, and probed with BIM antibody. (B) AnnexinV–propidium iodide (PI) staining of PC-9 cells after the BIM was silenced. Cells were seeded in a six-well plate, transfected with BIM-specific or control siRNA. 24 hours after the transfection, and the cells were treated with 1 μM Gefitinib for 48 hours, and then the cells were collected and apoptosis was analyzed by AnnexinV–PI staining.