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Phosphorylation of AKT and ERK and expression of AKT, ERK, and BIM in Gefitinib-resistant NSCLC cell lines after the treatment of different drug combinations. Tumor cells were seeded in six-well plates and treated with different drug combinations as indicated for 24 hours, then cells were lysed, and the lysate was subjected to SDS-PAGE, and probed with indicated antibodies.
