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. 2013 Mar;28(2):153–159. doi: 10.1089/cbr.2012.1297

FIG. 1.

FIG. 1.

The radiosensitivity in lung cancer cell H1299 with different p53 phenotypes. Cell models with different p53 phenotypes were established by introducing pCDNA3.1-p53 or pCDNA3.1–175 H; western blotting was used to confirm the expression of wt p53 and 175H (data not shown). (A) Cell counting kit-8 (CCK-8) assay was used to detect the short-term cell viability in parental H1299 cells; (B) CCK-8 assay was used to detect the short-term cell viability in H1299-p53 cells, *p<0.05, versus sham-irradiated (0 Gy); (C) CCK-8 assay was used to detect the short-term cell viability in H1299-175H cells; (D) Colony formation assay was used to detect the long-term dose–survival curve, and Multitarget click model of GraphPad Prism 5.0 (Systat Software) was used to fit cell survival curves. H1299: D0=1.764, Dq=2.977; H1299-175H: D0=1.695, Dq=2.312; H1299-P53: D0=1.407, Dq=1.199.