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. 2013 Jan 7;19(7-8):928–937. doi: 10.1089/ten.tea.2011.0700

FIG. 2.

FIG. 2.

Antioxidant supplementation prevents reactive oxygen species (ROS) induced loss of differentiation. ALP expression was measured after 6 days of culture in BM or OM and the antioxidant effect (Trolox 50 μM and D-mannitol 5 mM) was measured in two-model systems of oxidative stress induction: t-BHP (50 μM) (A) or peroxynitrite (30 μM) (B). All conditions were performed at the same time, and the conditions were split for interpretation purposes only. Data are expressed as total ALP activity normalized for cell numbers. Error bars represent standard deviation. Statistical analysis was performed using Student's t-test with a significance of p<0.05. Asterisks represent *p<0.05, **p<0.01, ***p<0.001. ns, not significant.