FIG. 3.

Effect of antioxidant supplementation on DNA damage accrual. Effect of antioxidants (D-mannitol 5 mM, Trolox 50 μM, and sodium selenite [NaSel] 100 nM) on DNA damage load (A, B). DNA damage load was assessed by quantifying the percentage of 53BP1-positive cells (A, C) and the number of 53BP1 foci per cell (B, D) either in the absence of oxidative damage inducer (A, B) or in the presence of the oxidizing agent t-BHP (50 μM) (C, D). All conditions were performed at the same time, and the conditions were split for interpretation purposes only. Error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA and Tukey's post-test with a significance of p<0.05. Asterisks represent *p<0.05, **p<0.01. ns, not significant.