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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: J Cell Physiol. 2013 Feb;228(2):313–321. doi: 10.1002/jcp.22109

Fig 4.

Fig 4

Identification of Site 1 in the Runx2P1 promoter by genomic DNaseI protection analysis. LM-PCR mediated DNaseI footprinting of intact nuclei reveals a stable genomic protein/DNA interaction domain, Site 1, in the proximal region of the P1 promoter in Runx2 null calvarial cells (−/−) and MC3T3 osteoblasts (MC). The lane on the left shows the DNaseI digestion pattern of naked DNA (N) and the right lane shows a ladder of DNA cleaved at G residues. The diagram at the bottom shows the location of Site 1 in relation to known transcription factor motifs in the Runx2 P1 promoter and the three nested LM-PCR primers used for the analysis (LM7-9).