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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: J Cell Physiol. 2013 Feb;228(2):313–321. doi: 10.1002/jcp.22109

Fig 5.

Fig 5

Proliferation-related genomic protein/DNA interactions at Site 2 in the Runx2 P1 promoter. Genomic LM-PCR assisted DNaseI protection analysis indicates that there is a second stable genomic protein/DNA interaction domain, Site 2, in the distal region of the Runx2 P1 promoter. The size of the DNaseI footprint in quiescent (Q) MC3T3 osteoblasts is reduced compared to proliferating (P) cells, indicating selective loss of protein/DNA interactions. The left and right parts show DNaseI digestion patterns for, respectively, the sense-strand (using primers LM1-3) and the anti-sense strand (using primers LM4-6). The left part shows two replicates each for Q and P samples. The left lane in both left and right parts shows the DNaseI cleavage patterns of naked DNA (N). The diagram at the bottom shows relevant transcription factor consensus motifs.