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. Author manuscript; available in PMC: 2013 Mar 6.
Published in final edited form as: Bone. 2011 Dec 28;50(4):954–964. doi: 10.1016/j.bone.2011.12.013

Figure 3.

Figure 3

HS5 and heparin, but not commercial HSs, prolonged BMP-2 stability and activity. (A) BMP-2 stability over time was measured in a BMP-2 Quantikine ELISA kit. 100 ng/mL of BMP-2 was incubated in the presence/absence of 3 μg/mL of GAG for up to 72 h. (B) BMP-2 incubated with heparin or HS5 for up to 72 h remained biologically active as indicated by its ability to induce Smad 1/5/8 phosphorylation (p-Smad 1/5/8) in C2C12 cells.