. Author manuscript; available in PMC: 2013 Nov 15.

Published in final edited form as: Anal Biochem. 2012 Aug 27;430(2):185–192. doi: 10.1016/j.ab.2012.08.021

Table 1.

Assay characteristics and statistics for the optimized detection of BoNT serotypes A, B, C, D, E, and F measured in buffer, milk, or serum

Assay	Capture Ab	Detection Ab	Matrix	LOD (pg/ml)	R ²	% Recovery^a	% Recovery^b
BoNT/A	AR4	RAZ1	Buffer	0.8	0.989	98.4	109.5
			Milk	0.8	0.993	93.9	116.3
			Serum	0.9	0.996	101.2	98.5
BoNT/B	B2	1B10.1	Buffer	0.5	0.996	99.3	85.5
			Milk	0.8	0.987	101.9	108.5
			Serum	0.8	0.995	95.8	97.8
BoNT/C	C1	1C1	Buffer	2.2	0.989	90.5	86.3
			Milk	2.7	0.978	93.2	84.5
			Serum	3.5	0.992	90.1	98.2
BoNT/D	8DC1	8DC2	Buffer	0.9	0.984	89.5	100.9
			Milk	1.1	0.990	98.4	97.2
			Serum	5.1	0.946	94.8	97.5
BoNT/E	3E6.2	3E4.1	Buffer	0.2	0.980	95.2	93.0
			Milk	0.2	0.978	92.3	96.7
			Serum	0.5	0.981	97.4	90.2
BoNT/F	6F8	6F5	Buffer	1.4	0.991	93.9	99.2
			Milk	2.2	0.972	101.2	95.2
			Serum	1.7	0.920	101.1	98.0

Percentage recovery of antigen added at a toxin spike level concentration of 313 pg/ml.

Percentage recovery of antigen added at a toxin spike level concentration of 20 pg/ml.