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. 2012 Dec;97(12):1904–1908. doi: 10.3324/haematol.2012.066399

Figure 2.

Figure 2.

Distribution and function of H2Rs on morphological subtypes of AML. H2R expression was analyzed by FACS on PBMC or bone marrow from newly diagnosed AML patients (n=26). (A) Percentage of myeloid cells (defined as CD33+ and/or CD34+) expressing H2Rs in cells from patients with AML of indicated FAB classes. (B) Median fluorescence intensity of H2R expression on immature blasts (CD33+ and/or CD34+, CD14- and CD15- myeloid cells) or mature CD14+ leukemic cells. Horizontal lines represent median values. (C) Fraction of CD33+ and/or CD34+ myeloid cells co-expressing H2R and gp91phox in patients with different morphological subtypes of AML. (D) H2R expression visualized by confocal microscopy on immature FAB-M2 cells, immature FAB-M4 cells, and mature FAB-M4 leukemic cells. Bars represent 5 μm. (E) Bars show extracellular ROS production, assayed in the presence or absence of histamine (100 microM) in bone marrow cells from patients with FAB-M4/M5 AML, evaluated using one-sample t-test. (F) Extracellular ROS production by FACS-sorted mature (CD14+) FAB-M4/M5 cells and its inhibition by histamine (P<0.001, one sample t-test). (G) NK cell apoptosis after overnight culture of NK cells from healthy donors with mature CD33+CD14+ FAB-M4/M5 AML cells. Data are from the first NK:AML cell ratio to reach 40% apoptosis (range 8:1 to 2:1; n=5). *P<0.05, **P<0.01, ***P<0.001.