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. Author manuscript; available in PMC: 2014 Jan 7.
Published in final edited form as: Mol Pharm. 2012 Sep 20;10(1):26–32. doi: 10.1021/mp300238w

Figure 4. Determining localization of CPMV with endosomal markers EEA1, Rab 5, and Rab 11.

Figure 4

(A–B) Hela cells incubated 2 hr with CPMV (green) and stained for EEA1 (red). White in B shows colocalized points. Amount of colocalization representative of all timepoints studies (30 min, 1 hr, 2 hr, and 4 hr). (C) Partial colocalization of internalizing CPMV with early endosomal marker Rab5. RAW264.7 macrophages incubated with CPMV (green) for 45 minutes and stained with Rab5 antibody (red). This timepoint showed the most colocalization (white, compared with 15, 30, and 60 minutes. (D) Partial colocalization of internalizing CPMV with recycling endosome marker Rab11. Hela cells were incubated with CPMV (green) for 3 hrs and stained with Rab11. Bar, 10 µm.