Figure 2. Damage assessment using linear imaging techniques.

a) Confocal, b) LT and c) combined images of the region surrounding the axon before the laser dissection. d–f) show the same region after the surgery (see Media S1). Damage is evidenced by increased autofluorescence in the confocal image and a dark spot in the LT image. Both damage structures colocalize at the combined image. Excitation of the GFP labeled neurons was done at 488 nm. Arrows point to the place of the laser axotomy. Scale bar 10 µm.