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. 2013 Mar 6;8(3):e58298. doi: 10.1371/journal.pone.0058298

Figure 5. Structures of AstC prior and after reaction with substrates.

Figure 5

Figure 5A shows the 2Fo-Fc density (blue mesh) for the PLP cofactor bound to Lys252 in a Schiff base. The B protomer is in green and the A protomer loop of 277–284 is shown in magenta. The electron density is set at 1.5 sigma. Figure 5B shows the density for PLP and Lys252 after the crystals were soaked with ORN. It is clear that the covalent bond between Lys252 and the PLP is broken and we have now modelled the PLP with an additional oxygen atom. Figure 5C shows the electron density for Lys252, PLP and the SO found in the SO soaked crystals. There is clean electron density that shows the SO covalently bound to the PLP and a break from Lys252. All density figures are 2Fo-Fc maps and were made with the ligands in the model.