Figure 4. Celastrol can significantly inhibit CCL2 expression both at transcriptional and protein level.

(A) VCaP cells were treated with Celastrol for 2 h, the inhibition of CCL2 at RNA level was shown in a dose-depedent manner by real-time PCR; (B) when Celastrol treatments were done for 24 h, even 0.05 µM can dramatically decrease CCL2 expression; (C) when exposed to Celastrol for 24 h, secreted CCL2 protein in culture medium by VCaP cells are significantly decreased. ELISA data are shown. Each concentration was tested in triplicate and the experiment was repeated three times. Asterisks indicate statistically significant differences between Celastrol treated group and the control by t-test.