Figure 5. Celastrol inhibit VCaP cell growth in vitro.
(A) Cells (1×105) were plated in 35 mm dishes in complete medium and treated with different concentration of Celastrol for 24 h. Proliferation of the 8 groups of VCaP cells were measured using a Coulter counter. Cells were trypsinized and counted in triplicate. Cell numbers for each group are divided by the total cell number in the control group without Celastrol treatment. The experiment was repeated three times. Mean +/− standard deviation is shown. Asterisks indicate statistically significant differences between Celastrol treated group and the control. (B) 1×105 of VCaP, LNCaP, PC3 and DU145 were exposured to 0.5 µM Celastrol for 24 h, cell numbers were counted for each group and divided by the total cell numbers in control groups to obtain the survival ratio.The experiment was repeated three times. Asterisks indicate statistically significant differences between different cell lines and the VCaP cells.