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. 2013 Mar 6;8(3):e58203. doi: 10.1371/journal.pone.0058203

Figure 2. Q-PCR quantification of artificial mixtures containing genomic DNA extracted from S. epidermidis and P. acnes or M. restricta in various ratios.

Figure 2

(A) quantification of P. acnes alone or in combination with S. epidermidis (ratios 1∶1; 1∶10; 1∶100 and 1∶1000) or M. restricta (ratios 1∶0.01; 1∶0.1; 1∶1 and 1∶10); (B) quantification of S. epidermidis alone or in combination with P. acnes or M. restricta in the same ratios and (C) quantification of M. restricta alone or in combination with P. acnes or S. epidermidis (ratios 1∶100; 1∶1000; 1∶10000 and 1∶100000). Q-PCR quantification showed the same cell count when the three microorganisms were analyzed alone or in a mixture with the other major skin contaminants.