Figure 2. Genotoxicity of B(a)P in Apc^+/+ and Apc^Min/+ cell lines.

Apc^+/+ and Apc^Min/+ cells were treated with the indicated concentrations of BaP for 24 h. (A) Genotoxicity was evaluated by HPLC-MS/MS quantification of dG-N²-BPDE in DNA. (B) Genotoxicity was evaluated with γHAX ICW assay. Bars represent the average of at least three independent experiments with SEM. Statistically significant increase in H2AX phosphorylation compared with DMSO control using Student’s test; *, p<0.05; **, p<0.01. Statistically significant difference between Apc^+/+ and Apc^Min/+ cells using Student’s test; b, p<0.05; a, p<0.01.