Table 2. Genotoxicity and cytotoxicity of B(a)P and PhIP in Apc+/+ and ApcMin/+ cell lines.
| Cell line | Apc+/+ | ApcMin/+ | ||||
| Treatment | DNAadducts/(108 nn) | γH2AX foldinduction | Viability% | DNAadducts/(108 nn) | γH2AX foldinduction | Viability% |
| PhIP 1 µM | nd | 1.07 (0.14) | 100 (1) | 13 (3) | 1.12 (0.11) | 96 (2) |
| PhIP 3 µM | nt | 1.11 (0.16) | 104 (4) | nt | 1.19 (0.08) | 97 (2) |
| PhIP 10 µM | 12 (1) | 1.16 (0.12) b | 98 (4) | 93 (20) | 1.43 (0.17) ** | 95 (1) |
| PhIP 30 µM | 32 (6) | 1.27 (0.19) *, b | 97 (4) | 561 (20) | 2.11 (0.41) ** | 81 (3) |
| PhIP 100 µM | 169 (60) | 1.53 (0.26) **, a | 88 (5) | 6108 (1000) | 2.68 (0.50) ** | 67 (2) |
| BaP 0.1 µM | 4 (2) b | 1.32 (0.11) ** | 104 (5) | 9 (1) | 1.29 (0.07) ** | 97 (3) |
| BaP 1 µM | 32 (6) b | 2.18 (0.29) ** | 94 (7) | 45 (4) | 2.41 (0.21) ** | 81 (3) |
| BaP 10 µM | 156 (24) a | 2.40 (0.33) **, a | 90 (7) | 331 (45) | 4.25 (0.56) ** | 67 (4) |
| BaP 1 µM/PhIP10 µM | 27 (1)/64 (10) a | 1.90 (0.30) | 94 (5) | 63 (13)/468 (23) | 2.15 (0.25) | 76 (2) |
nn : normal nucleosides.
nd : not detected.
nt : not tested.
Values are expressed as means (SEM), n≥3.
*
: Statistically significant increase in H2AX phosphorylation compared with DMSO control was determined using Student’s test (*, p<0.05; **, p<0.01); a, b: significant difference between Apc+/+ and ApcMin/+ cells using Student’s test (a, p<0.05; b, p<0.01).