Table 3. Tm of PNA:RNA 1 duplexes at varying salt concentrations.* .
| [NaCl] = | 0 M | 50 mM | 100 mM | 250 mM | 500 mM | 1 M |
| PNA nf:RNA 1 | 47.4±0.6 | 46.4±0.8 | 47.3±1.0 | 47.1±1.1 | 47.8±1.0 | 48.5±0.8 |
| PNA 1neg:RNA 1 | 48.3±0.5 | 46.9±1.3 | 47.4±1.3 | 48.5±1.3 | 49.1±1.3 | 53.5±1.3 |
| PNA 3neg:RNA 1 | 50.1±0.7 | 52.5±0.7 | 51.3±0.4 | 53.5±0.4 | 54.1±0.9 | 55.8±0.6 |
| PNA 1pos:RNA 1 | 54.7±1.3 | 46.9±1.4 | 48.3±1.5 | 49.5±1.3 | 51.1±1.2 | 52.1±1.0 |
| PNA 3pos:RNA 1 | 57.2±0.8 | 54.6±0.4 | 51.6±1.1 | 48.0±1.3 | 50.6±1.0 | 50.9±1.5 |
| PNA 1Me:RNA 1 | 50.5±1.3 | 50.1±1.1 | 50.6±1.1 | 51.6±1.1 | 53.3±0.9 | 54.0±1.0 |
*
Conditions: 3 µM PNA, 3 µM RNA, 10 mM sodium phosphate buffer with added NaCl, pH 7.2. Errors represent standard deviation of three or four independent trials.