Table 5. Thermodynamic parameters for the formation of duplexes of PNA 3neg and PNA 3pos with DNA and RNA.* .
| Duplex | Tm (°C) | −ΔG(kJ⋅mol−1) | −ΔH(kJ⋅mol−1) | −TΔS(kJ⋅mol−1) |
| PNA 3neg:DNA 1 | 46.1±0.6 | 48.0±0.3 | 208.4±1.0 | 160.4±1.0 |
| PNA 3pos:DNA 1 | 49.1±1.0 | 49.8±0.3 | 213.2±2.7 | 163.4±2.9 |
| PNA 3neg:RNA 1 | 49.9±1.5 | 49.1±0.8 | 203.4±3.6 | 154.3±2.9 |
| PNA 3pos:RNA 1 | 46.5±1.7 | 47.9±0.3 | 191.5±0.4 | 143.6±0.6 |
*
Averages from van't Hoff analysis of three trials of UV melting data. Errors represent standard deviation of three independent trials.