Figure 10. Competition assay using a mouse CD28 monoclonal antibody and purified human CD28 autoantibodies.

Jurkat cells were incubated with mixtures of mouse monoclonal CD28 antibodies with increasing amounts of purified human autoantibodies (CD28 or G250). Competition was detected for human CD28 autoantibodies while human G250 autoantibodies had no effect. Cell viability was measured by EZ4U assay.