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. 2013 Mar 6;8(3):e58462. doi: 10.1371/journal.pone.0058462

Figure 2. Expression analysis of gei-8.

Figure 2

(A) Schematic representation of the predicted gei-8a isoform consisting of 17 exons compared with detected expression. cDNA clones 7320, 7323 and 7324 are indicated with their exons (open rectangles). Expression of exon 12 and 16 is not constant. Exon 12 in cDNA clone 7323 and 45 bp from exon 16 in cDNAs 7323 and 7324 were removed by alternative splicing (bottom two lines). The location of predicted SANT and glutamine-rich interaction domains is marked by lines above the gei-8a diagram. Location of gei-8(ok1671) mutation used in expression analysis is marked by a line below the gei-8a diagram. Two regions identified as putative CoRNR nuclear receptor binding motifs are indicated in the exon 8 and 15 (NR). (B) Schematic representation of predicted gei-8 isoforms a, b and c. (C) GFP reporter gene constructs #1, #2 and #3 used for expression analysis. (D) Overlapping regions of gei-8 gDNA used for rescue. The size of the overlapping region is 191 bp.