Table 2. Induction of additional gonad and body shape phenotypes in homozygous gei-8(ok1671) mutant worms by RNAi directed against sqrd-2 or sqrd-1.

Target gene	Screening method	Hermaphrodites injected	Homozygous larvae scored	Larvae with additional gonad and body shape defects
sqrd-2	Nomarski optics	24	211	40
sqrd-2	DAPI staining	15	270	27
Total for sqrd-2	Nomarski optics+DAPI staining	39	481	67 (13.9%)
sqrd-1	Nomarski optics	30	151	5
sqrd-1	DAPI staining	10	47	4
Total for sqrd-1	Nomarski optics+DAPI staining	40	198	9 (4.5%)