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. 2013 Mar 6;8(3):e57266. doi: 10.1371/journal.pone.0057266

Figure 10. The sperm phenotypes from spe-46(hc197); him-5(e1490) males.

Figure 10

Males were dissected in SM1 buffer (unactivated), in SM1 plus Pronase (200 µg/ml) (pronase activated), and in SM1 plus 70 mM Triethanolamine (TEA) (TEA activated). Sperm phenotypes are categorized into: (i) spermatid (blue), (ii) spermatozoa (purple), (iii) sperm that appeared swollen (green), (iv) sperm that had large vacuoles (red), and (v) sperm with extended spikes (blue). The number is expressed as percent total sperm. DIC image of sperm phenotypes are shown at the bottom of the graph. Scale bar is 5 µm.