Skip to main content
. 2013 Mar 6;8(3):e58956. doi: 10.1371/journal.pone.0058956

Figure 6. Kar2 has a role in azole drug resistance downstream of the UPR pathway without affecting ERG11 and ERG3 expression.

Figure 6

(A) WT H99 strain, ire1Δ and hxl1Δ mutants, and PH3 :KAR2 strains (YSB1751, YSB1741, and YSB1745) were grown for 16 hr at 30oC in a liquid YPD medium, 10-fold serially diluted and spotted on a YPD agar medium containing the indicated concentrations of azole drugs and photographed. (B) The expression levels of ERG11 and ERG3 in strains described in (A). Each membrane was hybridized with an ERG11 or ERG3-specific probe, washed, and developed. Subsequently, the same membrane was stripped, subjected to re-hybridization with the ACT1-specific probe, washed, and developed. Expression levels of ERG11 or ERG3 were quantitatively measured with a phosphorimager and normalized with those of ACT1. Each KAR2/ACT1 is a value relative to that of the WT strain set to 1.0. (C) Strains described in (A) were grown for 16 hr at 30°C in a liquid YPD medium, 10-fold serially diluted, spotted on a YPD agar medium containing the indicated concentrations of SDS and fludioxonil, and photographed after incubation for 3 days.