Figure 8. Kar2 is not sufficient for Ire1-mediated regulation of capsule production in C. neoformans.
To measure the capsule production, each strain [WT H99 strain, the ire1Δ mutant (YSB552), and PH3 :KAR2 strains (YSB1741 and YSB1751)] was spotted and cultured on a DME agar medium at 30oC for 2 days. Capsules were visualized by India ink staining (A), and the relative capsule volume was measured by calculating the ratio of the length of the packed cell volume phase per length of the total volume phase (B). Three independent experiments with technical triplicates were performed. The scale bar represents 10 µm. Error bars represent the standard deviation.