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. 2013 Mar 6;8(3):e58560. doi: 10.1371/journal.pone.0058560

Figure 4. STXBP1 is not required for IκB-NFκB pathway activation, MAP kinase phosphorylation, or Egr and NFAT activity in FcεRI-activated mast cells.

Figure 4

A, STXBP1+/+ and STXBP1−/− mast cells were sensitized with anti-TNP IgE and stimulated with TNP-BSA (10 ng/ml) for various times. Whole cell lysates were analyzed by Western blotting for phospho-IκB and total IκB, phospho-p38 and total p38, phospho-JNK and total JNK, phospho-ERK1/2 and total ERK1/2, phospho-Akt and total Akt, Syntaxin-1, PKG-1, and Actin. B-D, STXBP1+/+ and STXBP1−/− mast cells were sensitized with anti-TNP IgE and stimulated with TNP-BSA (10 ng/ml) for various times. The nuclear proteins were isolated and subjected to EMSA. Egr binding consensus sequence (5′-GGA TCC AGC GGG GGC GAG CGG GGG CGA ACG-3′) (B), NF-κB binding consensus sequence (5′-TTA TCA AAT GTG GGA TTT TCC CAT-3′) from IL-6 promoter (C), and NFAT binding concensus sequence (5′-AAG GTG TTT CCC CAA GCC TTT CCC-3′) from IL-13 promoter (D) were labeled with 32P for EMSA. Shown is a representative from three independent experiments.