Table 2. Some RNA helicases studied by single-molecule approaches, in order of appearance in text.
Helicase | Full name (www.uniprot.org) | Subgroup | Biological role | Substrate | Method | Ref. |
---|---|---|---|---|---|---|
eIF4A |
eukaryotic initiation factor 4A |
DEAD-box |
translation initiation |
dsRNA |
AFM, smFRET |
80
,
111
|
Ded1 |
ATP-dependent RNA helicase DED1 |
DEAD-box |
translation initiation |
ssRNA, dsRNA |
AFM, smFRET |
80
,
140
|
HCV NS3 |
Hepatitis C virus non-structural protein NS3 |
DEAH-box |
viral replication |
dsRNA/DNA with 3′ ss overhang55,114 |
Optical tweezers, smFRET* |
112
,
115
,
118
,
119
|
NPH-II |
Nucleoside triphosphate phosphohydrolase II |
DEAD-box |
transcription termination, RNA export146 |
viral dsRNA |
smFRET |
121
|
Mss116 |
ATP-dependent RNA helicase MSS116, mitochondrial |
DEAD-box |
splicing |
group I and II introns |
smFRET |
124
|
RIG-I |
retinoic acid inducible gene I |
DExD/H-box |
RNA interference,176 immune response168 |
viral dsRNA |
PIFE |
135
|
YxiN | ATP-dependent RNA helicase YxiN | DEAD-box | ribosome biogenesis177 | 23S rRNA178 | smFRET | 126 - 129 |
Study performed using dsDNA.