Figure 2. Inactivation of the cshA gene results in a cold-sensitive growth phenotype. The parental wild type strain S30, the cshA::mini-Mu insertion, the mutant complemented with wild type cshA and a Walker A motif mutant (K52A) under the control of a xylose-inducible promoter were spotted in serial dilutions (indicated underneath) on rich medium containing 1% xylose and chloramphenicol (15 μg/ml). All were incubated at 42°C, 37°C, 30°C, and room temperature for 24 h, 24 h, 48 h, and 4 d, respectively. Plasmids expressing GFP instead of CshA were used as controls